Technologies

Technologies

The Chemical Biology Platform can employ a variety of techniques in various microtiter plate-based formats (96/384 wells). Assays can be conducted with either purified protein, membranes, living cells (mammalian, bacteria, yeast) using a variety of readouts, including: 

  • Radioactivity-based assays
    • Radioligand binding
    • Enzymatic and/or signal transduction assays
  • Fluorescence intensity
    • FRET
  • Fluorescence polarisation/anisotropy
     
  • Time-resolved fluorescence
    • Tr-FRET
  • Luminescence
    • Flash
    • Glow
    • BRET
    • Protein fragment complementation
  • Absorbance
     
  • Surface Plasmon Resonance
     
  • Isothermal titration calorimetry
     
  • Virtual screening technologies
    • Chemoinformatics analyses
    • Protein homology modeling
    • Pharmacophore modeling
    • Molecular docking simulations
    • Ligand binding site comparison

For most screening requests, these different technologies will offer suitable options to conduct a reliable screen.